For the past 25 years, scientists have been developing fluorescent proteins for bioimaging. Today their diversity covers nearly the whole visible spectrum, including blue, cyan, green, yellow, orange, red, far-red and near infrared, allowing scientists to produce detailed images of various proteins’ localization within live cells using multicolour labelling.
A team of researchers at Institut Laue–Langevin (ILL) have used neutron scattering experiments to study a family of fluorescent proteins called Cyan Fluorescent Proteins (CFPs). “CFPs are very useful tools for bioimaging as they can be used to produce pictures of live cells - if the CFP is used as a fusion protein, it can reveal information about protein location, and if used as part of a biosensor, it can report on the evolution of various cell parameters (pH, calcium concentration…)” says Dr Judith Peters, scientist at ILL and professor at Université Grenoble Alpes.